Part I - Directed Evolution

1 - Continuous Evolution of ProteinsIn Vivo
2 - In VivoBiosensors for Directed Protein Evolution
3 - High-Throughput Mass Spectrometry Complements Protein Engineering
4 - Recent Advances in Cell Surface Display Technologies for Directed Protein Evolution
5 - Iterative Saturation Mutagenesis for Semi-rational Enzyme Design

Part II - Rational and Semi-Rational Design

6 - Data-driven Protein Engineering
7 - Protein Engineering by Efficient Sequence Space Exploration Through Combination of Directed Evolution and Computational Design Methodologies
8 - Engineering Artificial Metalloenzymes
9 - Engineered Cytochrome P450s for Biocatalysis

Part III - Applications in Industrial Biotechnology

10 - Protein Engineering Using Unnatural Amino Acids
11 - Application of Engineered Biocatalysts for the Synthesis of Active Pharmaceutical Ingredients (APIs)
12 - Directing Evolution of the Fungal Ligninolytic Secretome
13 - Engineering Antibody-Based Therapeutics: Progress and Opportunities
14 - Programming Novel Cancer Therapeutics: Design Principlesfor Chimeric Antigen Receptors

Part IV - Applications in Medical Biotechnology

15 - Development of Novel Cellular Imaging Tools Using Protein Engineering