Satoshi Obika, Osaka University, Japan

Mitsuo Sekine, Tokyo Institute of Technology, Japan

Presents the latest knowledge on a range of topics relating to the synthesis of therapeutic oligonucleotides

Aims to provide novel insights and inspire fresh ideas in nucleic acid chemistry

Meets the needs of both established researchers and students new to the field

Part 1. Synthesis of Natural Oligonucleotides.- Chapter 1. Non-protected Synthesis of Oligonucleotides.- Chapter 2. Various Coupling Agents in the Phosphoramidite Method for Efficient Oligonucleotide Synthesis.- Chapter 3. Recent Development of Chemical Synthesis of RNA.- Chapter 4. RNA Synthesis Using the CEM Group.- Chapter 5. Liquid-Phase Synthesis of Oligonucleotides.- Chapter 6. Large-Scale Oligonucleotide Manufacturing .- Part 2: Synthesis and Properties of Artificial Oligonucleotides.- Chapter 1. Nucleosides and Oligonucleotides Incorporating 2-Thiothymine or 2-Thiouracil Derivatives as Modified Nucleobases.- Chapter 2. Site-Specific Modification of nucleobases in oligonucleotides.- Chapter 3. Four-Hydrogen-Bonding Base Pairs in Oligonucleotides: Design, Synthesis, and Properties.- Chapter 4. Photo-Cross-Linkable Artificial Nucleic Acid: Synthesis and Property of 3-Cyanovinylcarbazole-modified Nucleic Acids and Its Photo-induced Gene-Silencing Activity in Cells.- Chapter 5. Effects of 2¿-O-Modifications on RNA Duplex Stability.- Chapter 6. 2',4'-Bridged Nucleic Acids Containing Plural Heteroatoms in the Bridge Moiety.- Chapter 7. Synthesis and Therapeutic Applications of Oligonucleotides Containing 2'-O,4'-C-Ethylene- and 3'-O,4'-C-Propylene-Bridged Nucleotides.- Chapter 8. RNA Bioisosters: Chemistry and Properties of 4'-ThioRNA and 4'-SelenoRNA.- Chapter 9. Development of Triplex Forming Oligonucleotide including Artificial Nucleoside Analogues for the Antigene Strategy.- Chapter 10. Chemical Synthesis of Boranophosphate Deoxyribonucleotides.