Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Setting Up a Single-Cell Genomic Laboratory.- Tissue Handling and Dissociation for Single-Cell RNA-seq.- Full-Length Single-Cell RNA-Sequencing with Smart-seq2.- CEL-Seq2 - Single Cell RNA Sequencing by Multiplexed Linear Amplification.- Single-Cell RNA-seq by Multiple Annealing and Tailing Based Quantitative Single-Cell RNA-seq (MATQ-seq).- Single-Cell RNA Sequencing with Drop-Seq.- Chromium 10X Single Cell 3' mRNA Sequencing of Tumor Infiltrating Lymphocytes.- Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA-Sequencing.- Single-Cell Tagged Reverse Transcription (STRT-seq).- Single-Cell RNA-Sequencing of Peripheral Blood Mononuclear Cells with ddSEQ.- High Throughput Single Cell Real-Time Quantitative PCR Analysis.- Single-Cell Dosing and mRNA Sequencing of Suspension and Adherent Cells Using the Polaris(TM) System.- Targeted TCR Amplification from Single-Cell cDNA Libraries.- Sequencing the Genomes of Single Cells.- Studying DNA Methylation in Single Cell Format with scBS-seq.- Single Cell 5fC Sequencing.- ChIPmentation for Low-Input Profiling of In Vivo Protein-DNA Interactions.- Immuno-Phenotyping of Human Peripheral Blood Mononuclear Cells by Mass Cytometry.- Classification of the Immune Composition in the Tumor Infiltrate.- Combined Genome and Transcriptome (G&T) Sequencing of Single Cells.- Simultaneous Pofiling of mRNA Transcriptome and DNA Methylome from a Single Cell.- Simultaneous Targeted Detection of Proteins and RNAs in Single Cells.- CRISPR-Screening in Single Cells.- Single Cell Live Imaging.- Differential Expression Analysis in Single Cell Transcriptomics.- A Bioinformatic Toolkit for Single-Cell mRNA Analysis.