Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Introduction: Plant Cold Acclimation and Winter Survival.- Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays.- Differential Thermal Analysis: A Fast Alternative to Frost Tolerance Measurements.- Infrared Thermal Analysis of Plant Freezing Processes.- Conducting Field Trials for Frost Tolerance Breeding in Cereals.- A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes.- Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation.- Identification of Arabidopsis Mutants with Altered Freezing Tolerance.- Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues.- Using Pixel-Based Microscope Images to Generate 3-D Reconstructions of Frozen and Thawed Plant Tissue.- Phenotyping Plant Cellular and Tissue Level Responses to Cold with Synchrotron-Based Fourier-Transform Infrared Spectroscopy and X-Ray Computed Tomography.- Proteomic Approaches to Identify Proteins Responsive to Cold Stress.- Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins.- A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition.- Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry.- Determining the ROS and the Antioxidant Status of Leaves during Cold Acclimation.- Analysis of Changes in Plant Cell Wall Composition and Structure during Cold Acclimation.- Subcellular Compartmentation of Metabolites Involved in Cold Acclimation.- Mathematical Modeling of Plant Metabolism in a Changing Temperature Regime.- Characterization of Ice Binding Proteins from Sea-Ice Microalgae.- Isolation and Characterization of Ice-Binding Proteins from Higher Plants.