Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Fast Masking Of Repeated Primer Binding Sites In Eukaryotic Genomes.- Primer Design for PCR Reactions in Forensic Biology.- Design of Primers and Probes For Quantitative Real-Time PCR Methods.- Large-scale Nucleotide Sequence Alignment And Sequence Variability Assessment To Identify the Evolutionarily Highly Conserved Regions For Universal Screening PCR Assay Design: An Example Of Influenza A Virus.- Low-concentration Initiator Primers Improve The Amplification Of Gene Targets With High Sequence Variability.- Multiplex PCR Primer Design for Simultaneous Detection of Multiple Pathogens.- Degenerate Primer Design for Highly Variable Genomes.- Allele-specific Real Time Polymerase Chain Reaction As A Tool For Urate Transporter 1 Mutation Detection.- MultiPLX: Automatic Grouping And Evaluation Of PCR Primers.- In silico PCR Primer Designing and Validation.- Primer Design using Primer Express® for SYBR Green Based Quantitative PCR.- Designing Primers for SNaPshot Technique.- Rapid and Simple Method of qPCR Primer Design.- PRIMEGENSw3: A Web-based Tool for High-throughput Primer and Probe Design.- Selecting Specific PCR Primers with MFEprimer.