Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Using FlyBase, a database of Drosophila genes and genetics.- The Q-System: A versatile repressible binary expression system.- Resources and methods for the analysis of microRNA function in Drosophila.- Analysis of single-cell transcriptome data in Drosophila.- Prime editing for precise genome engineering in Drosophila.- CRISPR/Cas9 mediated precise and efficient genome editing in Drosophila.- Tissue-specific CRISPR-Cas9 screening in Drosophila.- CRISPR-based transcriptional activation in Drosophila.- Tracing and manipulating Drosophila cell lineages based on CRISPR: CaSSA and CLADES.- Studying protein function using nanobodies and other protein binders in Drosophila.- Anchor Away: A system for fast inhibition of proteins in Drosophila.- Tagging Drosophila proteins with genetically-encoded fluorophores.- Optogenetic methods to control tissue mechanicsin Drosophila.- Optochemical control of cell contractility in Drosophila embryos.- Stiffness measurement of Drosophila egg chambers by atomic force microscopy.- Cultivation and live imaging of Drosophila imaginal discs.- Sample preparation and imaging of the pupal Drosophila abdominal epidermis.- FlyClear: A tissue clearing technique for high-resolution microscopy of Drosophila.- Preparation of Drosophila tissues and organs for transmission electron microscopy.- Segmentation and quantitative analysis of epithelial tissues.- Genetically-encoded sensors to study metabolism in Drosophila.