Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

1. Efficient Homologous Recombination Mediated in Planta Gene Targeting by Egg-cell Specific Expression of Staphylococcus aureus Cas9 from Arabidopsis.- 2. Rice gene knockout or down regulation through CRISPR-Cas9.- 3. Simultaneous CRISPR/Cas-mediated Single Base Editing in Rice Plant at More than One Locus .- 4. CRISPR-Cas9 Mediated Genome Editing of the Model Grass Species Brachypodium distachyon.- 5. CRISPR/Cas9-mediated Gene Editing of the Plant Pathogenic Oomycete Phytophthora palmivora.- 6. An effective CRISPR/Cas9 technology for efficiently isolating positive transformants and transgene-free mutants in a wide range of plant species.- 7. An optimized RNA-guided Cas9 system for efficient simplex and multiplex genome editing in barley.- 8. Genome editing of mammalian cells using CRISPR/Cas: From in silico designing to in-culture validation.- 9. Cloning free (DNA-free) CRISPR/Cas9-mediated gene knockout in human liver cell line and its detection.- 10. A procedure to design guideRNA, assembly fragments, and detect mutation for genome editing in flax.- 11. Delivery of Cas9/sgRNA RNP into wheat microspores using synthetic CPP for genome editing and gene expression modulation.- 12. CRISPR- Cas9 mediated gene editing in wheat: a step by step protocol.- 13. CRISPR-Cas9 based genome editing of banana.- 14. Homology-directed transgene-free gene editing in Chlamydomonas reinhardtii.